제품 상세
이종발효성 유산균 (heterofermentive lactic acid bacteria) 및 고농도의 thiamine을 요구하는 기타 균주를 배양하기 위한 고체 배지
Typical Formula*
Casein Peptone 12.5 g
Dextrose 10.0 g
Yeast Extract 7.5 g
Potassium Phosphate 5.0 g
Sodium Chloride 5.0 g
Sodium Citrate 5.0 g
Magnesium Sulfate 0.8 g
Polysorbate 80 0.2 g
Manganous Chloride 0.14 g
Ferrous Sulfate 0.04 g
Thiamine Hydrochloride 0.001 g
Agar 15.0 g
pH 6.7 ± 0.2 @ 25℃
* Adjusted as required to meet performance standards
Direction
Suspend 61.2 g of medium in 1000 ml of demineralized water. Heat to boiling with agitation to completely dissolve. Sterilize by autoclaving at 121℃ for 15 minutes or following established laboratory procedures. Dispense into appropriate containers.
Quality Control
Control | Incubation | Results |
Lactobacillus johnsonii ATCC® 33200 | Aerobic, up to 48 h @ 33-37℃ | Growth |
Lactobacillus rhamnosus ATCC® 9595 | Aerobic, up to 48 h @ 33-37℃ | Growth |
Lactococcus lactis ATCC® 19257 | Aerobic, up to 48 h @ 33-37℃ | Growth |
Lactococcus mesenteroides ATCC® 17071 | Aerobic, up to 48 h @ 33-37℃ | Growth |
Limitations
Thiamine is heat-labile; avoid excessive heating of medium. APT Agar is nonselective; coliforms and many common bacteria other than Lactobacillus will grow.